810105p  (Croda International Plc)

Journal: iScience

Article Title: M- O -M mediated denaturation resistant P2 tetramer on the infected erythrocyte surface of malaria parasite imports serum fatty acids

doi: 10.1016/j.isci.2024.109760

Figure Lengend Snippet:

Article Snippet: NBD-Palmitic acid , Avanti Polar , Cat# 810105P.

Techniques: Recombinant, Protease Inhibitor, Membrane, Mutagenesis, Virus, Software

Journal: bioRxiv

Article Title: SPNS1 is required for the transport of lysosphingolipids and lysoglycerophospholipids from lysosomes

doi: 10.1101/2022.12.14.520377

Figure Lengend Snippet: A , Illustration for postnatal deletion strategy of Spns1 using Rosa26Cre-ER T2 mice. B , Heatmap of lysoglycerophospholipids from livers of controls and gSpns1-cKO mice. n=5 mice per genotype. C-H , Total levels of lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), lysophosphatidic acid (LPA), lysophosphatidylglycerol (LPG), lysophosphatidylinositol (LPI), lysoplasmalogens (lyso-P). Each symbol represents one mouse. I , Heatmap of sphingolipids from livers of controls and gSpns1-cKO mice. n=5 mice per genotype. J , Total levels of sphingosines from livers of control and gSpns1-cKO mice. n=5 mice per genotype. K , Total levels of ceramides from livers of control and gSpns1-cKO mice. n=5 mice per genotype. L , Illustration of NBD-palmitate labeling experiment. M , Thin layer chromatography analysis of NBD-labelled phospholipids after 24 hours of pulse-labeling with NBD-palmitate in WT and Spns1-KO CHO cells. Arrowhead indicates NBD-LPC band. NL, neutral lipids. N , Quantification of NBD-LPC from the TLC plate. *p<0.05; ***p<0.001; ****p<0.0001; ns, not significant. Data are expressed as mean ± SD. Statistical significance was determined by two-sided unpaired t-test.

Article Snippet: For NBD-palmitate labeling, 10 μM NBD-palmitate (Avanti, 810105P) solubilized in 12% BSA was added to the cells for 24 hours in the growth medium.

Techniques: Labeling, Thin Layer Chromatography